Loading
Sections

Skip to content. | Skip to navigation

You are here: Home

WELCOME TO THE UNC FLOW CYTOMETRY CORE FACILITY

The UNC Flow Cytometry Facility provides state-of-the-art flow cytometry and related services to the entire UNC-CH research community as well as to others in the Research Triangle Park area. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves at reduced cost. A major part of our mission is to teach this technology to investigators, students, and staff. Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data. The core is located on the 6th floor of the Mary Ellen Jones Building, 116 Manning Drive, Chapel Hill, NC.

INSTRUMENTATION

Analytic Cytometers Cell Sorting
Three 9-color Beckman CyAns Beckman MoFlo
18-color BD LSR II with HTS Module Beckman MoFlo XDP
Cytek-modified 5-color FACS Calibur in BSL-2 Containment
iCyt/Sony Reflection with BSL-2 sorting capability

Coming soon: Stratedigm S1000Ex for Microparticle analysis

Coming soon: FACS ARIA SORP also in BSL2 containment

Now available to UNC investigators: Amnis Image Stream Core at Duke University. Click here for more information.

Did you know?

The Flow Cytometry Core Facility hosts a number of analysis software options including specialty software on our Analysis computer in room 618 MEJB and access to our FlowJo site license. See our web page https://www.med.unc.edu/flowcytometry/software-for-acquisition-and-analysis/available-software or ask our staff for more information!

Upcoming Events

Wednesday, May 16, 2012 - High Speed Imaging Flow Cytometry. 11:30 - 12:30 PM Room 321 Jones Bldg, Duke University. Amnis cytometry systems (ImageStream and FlowSight) combine high-speed image capture with image quantification to create a statistically powerful microscopy platforms, enabling robust discrimination of cells based on their appearance.  The platforms enable new methods of research and improve traditional flow and imaging based applications. If attending the seminar or wish to demo FlowSight, please RSVP Ed Landers at  Edward.Landers@merckgroup.com

Flow Facility Introductory Training - June 6, 2012, Room 804 Mary Ellen Jones Bldg, 1:30- 4 PM. This 2-1/12 hour lecture serves to introduce users to the principles of flow cytometry and the core facility. Participants are encouraged to review basic tutorials listed in our 'Useful Flow Cytometry Links' page, prior to their training. Please email the director to sign up: ncfisher@med.unc.edu. A training fee applies to this lecture. See rates listed on our 'Rates-Policies-Rules' link.

June 9-15, 2012, 35th Annual Course in Flow Cytometry: Research Methods and Applications. Bowdoin College, Brunswick, Maine. http://www.vsh.com/bowdoin/ The annual research course will provide you with state-of-the-art information on important cutting-edge topics in cytometry. Nationally recognized instructors organize hands-on laboratories and present related lectures. If you’re interested in learning about cytometry or just seeing what’s new, come on and experience an intense week of hard work and good times in the great state of Maine.

Save the date: March 21-22, 2013 - The South East Flow Cytometry Interest Group (SEFCIG) will be hosted by UNC and the Research Triangle Cytometry Association at the Carolina Inn here in Chapel Hill!!

What's New!

See our updated Sorting Tips: A Guide for Investigators, with detailed information that you should know while planning your sorting experiment and communicating with our staff.

Are you planning on publishing your Flow Cytometry Data? New publication requirements are coming (or are already enforced) for many high impact journals. For a description of MIFlowCyt - 'Minimum Information for Flow Cytometry experiments', view this link: http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20623/pdf For now, see our link under 'Application Protocols' for instructions on running Calibration Beads.

The UNC Flow Cytometry Core Facility is supported in part by an NCI Center Core Support Grant (P30CA06086) to the UNC Lineberger Comprehensive Cancer Center.