Becton-Dickenson LSR II
The B-D LSR II represents the pinnacle of current flow cytometry technology.  Our particular LSR II is configured to provide up to 18 colors of fluorescence detection simultaneously permitting not only high order multi-parameter analysis but also flexibility in experimental design.   It is also closely matched to the laser excitations provided on the iCyt Reflection sorter to permit non-sorter experimental optimization without using the more expensive sorters.  The LSR II provides 7 lasers for optimizing the excitations required by a multitude of fluorochromes (see below).  Also, on the LSR II will be a high throughput sampling system.  This system can sample from 96- or 384-well plates.  Analysis of all 96 wells from a 96-well plate can be done in as little as 15 minutes (actual time will depend on a number of user selectable parameters).  Carryover between samples is typically less than 1% (based on beads - cells may require other conditions to achieve a like or better result).  The B-D LSR II is a fully digital platform.  Data from the detectors, following pre-amp processing, is sent to high resolution ADCs for linear and log display.

The LSR II will be available to be operated by Facility staff or by investigator, but will be made available initially only to those investigators who will utilize its specific capabilities.  Please discuss with the Facility Director any anticipated needs you may have for the LSR II.
 
Instrument Overview

Location:  31-338 Lineberger Bldg.
Laser Excitations: 355nm, 405nm, 488nm, 532nm, 561nm, 592nm, and 640nm
18 colors of fluorescence
FSC detection via photodiode or PMT for increased sensitivity
96/384-well high throughput automated sampling

Excitations - Possible Fluorochromes

UV/355nm excitation (2 detectors)- Hoechst, indo-1
violet/408nm (5 detectors)- Pacific Blue, Pacific Orange, DAPI, Violet DyeCycle, Quantum Dots
Live/Dead violet, Quantum Dots, CFP
blue/488nm (2 detectors) - FITC, AlexaFluor 488, GFP, YFP, PerCP, PerCP-Cy5.5
green/532nm (5 detectors)- PE, PE-TxRed, AlexaFluor 532, PE-Alexa610, PE-Cy5,
 PE-Cy5.5, PE-Alexa700, PE-Alexa 750, PE-Cy7, PE-Alexa 647
yellow/561nm (5 detectors)- PE, PE-TxRed, AlexaFluor 568, PE-AlexaFluor 610, PE-Cy5, PE-Cy5.5, PE-AlexFluor-700, PE-AlexaFluor 750, PE-AlexaFluor 647, DsRed, dTomato
orange/594nm (1 detector)- mCherry, mRFP, max FP-Red, AlexaFluor 594, Texas Red
red/640nm (3 detectors)- APC, APC-AlexaFluor 700, APC-Cy7, APC-AlexaFluor 750, AlexaFluor-700, AlexaFluor-647