| TRAINING |
A primary mission of the Flow Facility is to educate users in the proper performance of flow cytometry experiments, in the proper operation of the analytic flow cytometers, and in the proper analysis of flow cytometry data. To initiate this training, we first have users who wish to operate the cytometers attend a 2 part training course. The primary purpose of the first part of the course (lecture format) is to acquaint the users with the basics of how a flow cytometer works and, especially, what is going on inside "the box" when the user activates various functions. A basic understanding of how a flow cytometer works is critical to producing high quality data. This is followed by a hands-on small group (2-4) presentation on how to use the software and the particular instrument desired. A separate second part is required for each different machine (CyAn, FACScan, FACSCalibur, LSRII) the user wishes to use. Users must be trained by Facility staff. Training by others in the user's laboratory is not permitted. Other training is provided in the form of protocols and tutorials in other areas of this website. Part 1 of the training (a 2.5 hour lecture) is provided about every 6-8 weeks or sooner when we have 10+ registrants. The second part (2-3.5 hours) is scheduled with users at their convenience. To sign up for the next course please call the Facility at 6-1530 or email us your request and include your name, PI name, and your phone number. We will put you on the list for the next class and call you when it is scheduled. Training for other aspects of conducting flow cytometry experiments will be undertaken in individual discussions with users during the initial help sessions that are included as part of the training course. In addition, there will be useful information in the from of protocols and tutorials available on the website. We are planning a future comprehensive flow cytometry course if there is interest.
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